8-(1H-Tetrazol-5-yl)-11H-pyrido[2,1-b]quinazolin-11-ones and method of treating bronchial asthma using them

ABSTRACT

The present invention relates to 8-(1H-tetrazol-5-yl)-11H-pyrido[2,1-b]quinazolin-11-ones having the following structure: ##STR1## wherein R 1  and R 2  are hydrogen, alkyl, alkyloxy, halogen, hydroxy and methylenedioxy; R 3  is carboxamido, cyano or 5-tetrazolyl groupings and the pharmaceutically acceptable salts of acids and bases thereof. 
     These compounds exhibit anti-allergic properties and are useful as anti-asthmatic agents.

The present invention relates to 8-(1H-tetrazol-5-yl)-11H-pyrido[2,1-b]quinazolin-11-ones having the following structural formula: ##STR2## wherein R₁ and R₂ are hydrogen, alkyl, alkyloxy, halogen, hydroxy and methylenedioxy; R₃ is carboxamido, cyano or 5-tetrazolyl groupings and the pharmaceutically acceptable salts of acids and bases thereof.

In the above definitions for R₁ - R₃, alkyl and the alkyl portion in alkyloxy are meant to have 1 - 4 carbon atoms, e.g., methyl, ethyl, propyl, isopropyl, butyl and isobutyl. The term "halogen" includes all the four members, i.e., fluorine, chlorine, bromine, and iodine.

The preferred compounds of the present invention falling within the above generic description are:

R₁ r₂ =h and R₃ = 5-tetrazolyl

R₁ = 2--och₃, r₂ = h, r₃ = 5-tetrazolyl

R₁ = 2--ch₃, r₂ = h, r₃ = 5-tetrazolyl

R₁, r₂ =2,3-di-OCH₃, R₃ = 5-tetrazolyl

R₁ = 2--oh, r₂ = h, r₃ = 5-tetrazolyl

The compounds of this invention having the generic formula I above, have been found to reduce allergic responses to antigen challenge by inhibiting antibody-antigen reactions in mammals such as rats. When tested in accordance with the procedure of Herzig [Immunopharmacology, M. E. Rosenthale and H. C. Mansmann, Eds., John Wiley and Son, N.Y., 1975], these compounds inhibit allergic responses or passive cutaneous anaphylaxis (PCA) in the rat when administered orally, parenterally or by aerosol at a dose of 0.001-20 mg/kg. Accordingly, these compounds are indicated in the management of allergic conditions such as bronchial asthma. Generally speaking, in an adult human, a dose of 0.001-20 mg/kg orally, parenterally or by aerosol administration 1 to 3 times daily is suggested for the relief of bronchial asthma. As with anti-allergy treatment, the dose is to be adjusted for individual needs but is within the above range.

The compounds of this invention are more potent and orally effective over previously known compounds such as cromolyn sodium.

Thus, for example, cromolyn sodium in the aforesaid PCA test was found to be orally inactive whereas the instant compounds were found to be orally active. Parenterally, the instant compounds were also found to be more potent than cromolyn sodium.

According to the present invention, the aforesaid Compound I is prepared in three steps:

An approximately substituted anthranilic acid is condensed with one equivalent of 6-chloronicotinamide in acetic acid to give the corresponding substituted pyrido[2,1-b]quinazolinone-8-carboxamide intermediate. The carboxamide is dehydrated to the nitrile derivative using p-toluenesulfonyl chloride in pyridine and DMF. The tetrazolyl product is obtained from the nitrile using NaN₃, NH₄ Cl and DMF.

The corresponding salts are prepared by reacting the parent compound with an appropriate base, e.g., sodium bicarbonate, potassium bicarbonate or an acid, e.g., hydrochloric, hydrobromic, hydrosulfuric, by methods well-known in the art.

To further illustrate the practice of this invention, the following examples are included:

EXAMPLE 1 8-Carboxamido-2-methoxy-11-oxo-11(1H)-pyrido[2,1-b] quinazoline.

A mixture of 5-methoxy-anthranilic acid (27.0 g, 161 mmol), 6-chloro-nicotinamide (25.0 g, 160 mmol) and ethanol (500 ml) containing 15 ml of concentrated HCl was heated at reflux for 24 hours. The mixture was cooled to 0° and the resultant solid precipitate was collected by filtration to give 34.0 g (69.5%) of crude hydrochloride salt. This material was recrystallized twice from pyridine to give the analytical sample, m.p. 329°-32° dec.

EXAMPLE 2 8-Cyano-2-methoxy-11-oxo-11(H)-pyrido[2,1-b]quinazoline

A solution of 1.2 1. pyridine, 300 ml of DMF, 7.46 g (39.4 mmol) of p-toluenesulfonyl chloride and 7.45 g (27.6 mmol) of 8-carboxamido-2-methoxy-11-oxo-11(H)-pyrido[2,1-b]quinazoline was heated at 100° for 42 hours. The mixture was cooled and poured into 4 1. ice/H₂ O and acidified to pH 1 with concentrated HCl. The solid which formed was collected; yield, 5.0 g (72.2%), m.p. 273°-280° dec. The analytically pure nitrile was obtained after one recrystallization from pyridine, m.p. 281°-285° dec.

EXAMPLE 3 2-Methoxy-8-(1H-tetrazol-5-yl)-11-oxo-11(H)pyrido[2,1-b]-quinazoline.

A mixture of 3.00 g (12 mmol) of 8-cyano-2-methoxy-11-oxo-11(H)-pyrido[2,1-b]quinazoline, NaN₃ (2.22 g, 34.2 mmol), NH₄ Cl (1.83 g, 34.2 mmol) and 250 ml of DMF was heated at 115° for 20 hours. The mixture was cooled, poured onto 1.5 1. of ice/H₂ O and acidified with concentrated HCl. The solid which formed was collected; yield, 2.93 g (83%), m.p. 279°-299° dec. The sample of analytical purity was obtained by recrystallization from pyridine; yield, 2.25 g (64%), m.p. 302°-304° dec.

Other compounds of the present invention are prepared in an analogous manner.

The physical characteristics of the compounds of the present invention are set out in the following Table:

                                      TABLE                                        __________________________________________________________________________      ##STR3##                                                                                                        Elemental                                                                            Solvent of                             R.sub.4   R.sub.5 m.p. ° dec.                                                                   Formula   Analysis                                                                             Recryst.                               __________________________________________________________________________     H         8-CONH.sub.2                                                                           338-44                                                                               C.sub.13 H.sub.9 N.sub.3 O.sub.2                                                         CHN   pyridine                               H         8-CN    249-51                                                                               C.sub.13 H.sub.7 N.sub.3 O . 1/4H.sub.2 O                                                CHN   pyridine                               H         8-(5-tetrazolyl)                                                                       293-96                                                                               C.sub.13 H.sub.8 N.sub.6 O . 1/4C.sub.2 H.sub.6                                          CHN   EtOH                                   2-CH.sub.3                                                                               8-CONH.sub.2                                                                           332-36                                                                               C.sub.14 H.sub.11 N.sub.3 O.sub.2                                                        CHN   pyridine                               2-CH.sub.3                                                                               8-CN    307-09                                                                               C.sub.14 H.sub.9 N.sub.3 O                                                               CHN   pyridine                               2-CH.sub.3                                                                               8-(5-tetrazolyl)                                                                       284-86                                                                               C.sub.14 H.sub.10 N.sub.6 O.sub.2                                                        CHN   pyridine                               2-OCH.sub.3                                                                              8-CONH.sub.2                                                                           329-32                                                                               C.sub.14 H.sub.11 N.sub.3 O.sub.2                                                        CHN   pyridine                               2-OCH.sub.3                                                                              8-CN    281-85                                                                               C.sub.14 H.sub.9 N.sub.3 O.sub.2                                                         CHN   pyridine                               2-OCH.sub.3                                                                              8-(5-tetrazolyl)                                                                       302-04                                                                               C.sub.14 H.sub.10 N.sub.6 O.sub.2                                                        CHN   pyridine                               3-OCH.sub.3                                                                              CN      273-75                                                                               C.sub.14 H.sub.9 N.sub.3 O.sub.2                                                         CHN   pyridine                               3-OCH.sub.3                                                                              8-(5-tetrazolyl)                                                                       284-87                                                                               C.sub.14 H.sub.10 N.sub.6 O.sub.2                                                        CHN   pyridine                               2,3-di OCH.sub.3                                                                         CONH.sub.2                                                                             313-18                                                                               C.sub.15 H.sub.13 N.sub.3 O.sub.4 . 1/2H.sub.2                                           CHN   pyridine                               2,3-di OCH.sub.3                                                                         CN      309-12                                                                               C.sub.15 H.sub.11 N.sub.3 O.sub.3 . 1/2H.sub.2                                           CHN   pyridine                               2,3-di OCH.sub.3                                                                         8-(5-tetrazolyl)                                                                       300-303                                                                              C.sub.15 H.sub.12 N.sub.6 O.sub.3                                                        CHN   pyridine                               2,3-methylenedioxy                                                                       CONH.sub.2                                                                             368-73                                                                               C.sub.14 H.sub.9 N.sub.3 O.sub.4                                                         CHN   pyridine                               2,3-methylenedioxy                                                                       CN      319-23                                                                               C.sub.14 H.sub.7 N.sub.3 O.sub.3                                                         CHN   pyridine                               2,3-methylenedioxy                                                                       8-(5-tetrazolyl)                                                                       310-13                                                                               C.sub.14 H.sub.8 N.sub.6 O.sub.3                                                         CHN   pyridine                               2-Cl      CONH.sub.2                                                                             326-30                                                                               C.sub.13 H.sub.8 ClN.sub.3 O.sub.2                                                       CHNCl pyridine                               2-OH      CONH.sub.2                                                                             346-52                                                                               C.sub.13 H.sub.9 N.sub.3 O.sub.3                                                         CHN   pyridine                               __________________________________________________________________________ 

What we claim is:
 1. A compound of the formula: ##STR4## wherein R₁ and R₂ are hydrogen, halogen, hydroxy, methylenedioxy or lower alkyl or alkoxy of 1 to 4 carbons; R₃ is 5-tetrazolyl and the pharmaceutically acceptable salts thereof.
 2. A compound according to claim 1 in which R₁ and R₂ are hydrogen and R₃ is 8-(5-tetrazolyl).
 3. A compound according to claim 1 in which R₁ is 2--CH₃, R₂ is hydrogen and R₃ is 8-(5-tetrazolyl).
 4. A compound according to claim 1 in which R₁ is 2--OCH₃, R₂ is hydrogen and R₃ is 8-(5-tetrazolyl).
 5. A compound according to claim 1 in which R₁ is 2--OCH₃, R₂ is 3-OCH₃ and R₃ is 8-(5-tetrazolyl).
 6. A compound according to claim 1 in which R₁ and R₂ together is 2,3-methylenedioxy and R₃ is 8-(5-tetrazolyl).
 7. A method for the treatment of bronchial asthma in a mammal suffering from asthma which comprises the administration of an anti-asthmatically effective amount of a compound according to claim 1 at an adult human dose of 0.001-20 mg/kg orally, parenterally, or by aerosol. 